中国比较医学杂志2011，Vol.21Issue(12)：1-4,17,5.DOI:10.3969/j.issn.1671.7856.2011.012.001
甲型H1N1流感病毒感染不同免疫缺陷小鼠的比较
Comparison of Different Immunodeficient Mouse Strains Infected with Influenza A(H1N1)Virus
摘要
Abstract
Objective The functional status of the host immune system plays an important role in viral infection. The aim of this work was to study the differences among four immunodeficiency strains of mice and two wide-type mouse strains after infection of influenza A (H1N1) ( A/California/7/2009 (CA7)) virus. Methods The six inbred laboratory strains of mice were anesthetized by inhalation of ether and inoculated intranasally with 0. 05 mL of infectious virus H1N1 (CA7) suspension. The body weight loss kinetics, survival rates and pathological changes in the lung tissue after infection were examined. Results BALB/c mice and four immunodeficiency strains of mice showed high susceptibility in the viral infection compared with C57BL/6 mice. Half of C57BL/6 mice survived, while the mortality rates of the remaining five strains were 100% . C57BL/6 mice exhibited diffuse interstitial pneumonia in the initial stages of infection, and the lesions were gradually restricted to the late stage. BALB/c mice and four immunodeficiency strains of mice showed severe diffuse interstitial pneumonia, bronchial epithelial degeneration and necrosis, and there were significantly less inflammatory cells than that in the C57BL/6 mice. Conclusions Innate immunity and adaptive immunity play a major role at the early and late stages of the infection of influenza A (H1N1) virus. The state of the host immune system affects the infection and prognosis of influenza A (H1N1) virus infection.关键词
H1N1甲型流感病毒,感染/免疫缺陷/Scid小鼠/NOD/LtJ小鼠/BALB/c-nu小鼠/NOD-Scid小鼠Key words
Influenza A (H1N1) virus, infection/ Immune deficiency/ SCID mouse/ NOD/LU mouse/ BALB/cnu mouse/ NOD-SCID mouse分类
医药卫生引用本文复制引用
刘颖,邓巍,徐艳峰,朱华,马春梅,李枫棣,鲍琳琳,秦川..甲型H1N1流感病毒感染不同免疫缺陷小鼠的比较[J].中国比较医学杂志,2011,21(12):1-4,17,5.基金项目
中国医学科学院医学实验动物研究所基本科研业务费:DWS200904 （）
北京市科委项目:Z09000700090904 （）
中医药行业科研专项:200907001-5. （）
