中国比较医学杂志2011，Vol.21Issue(12)：56-60,5.DOI:10.3969/j.issn.1671.7856.2011.012.014
气管插管法接种H5N1病毒感染恒河猴及系统组织病毒检测
Endotracheal Intubation Inoculation of H5N1 Virus and Detection of Virus in Organ Tissues in the Rhesus Macaques
摘要
Abstract
Objective To evaluate the feasibility of endotracheal intubation route in preparation of H5N1 virus infection in rhesus macaques and to obtain a more sensitive animal model. Methods The rhesus macaques were infected with H5N1 avian influenza virus by endotracheal intubation. Clinical signs were assessed daily, and throat swab, nasal wash , major organs and blood were collected for detection of virus load, isolation and blood routine analysis. Results After infection the animals exhibited anorexia, lethargy and transient fever, and the total number of circulating leukocytes and lymphocytes was decreased. Virus isolation, RT-PCR assay of the throat swabs, nasal washs materials showed that the intestine, lung, brain, spleen, kidney, heart and liver were the target of the virus replication and the virus could replicate in organs outside the lung. In addition, virus was also detected in the blood 3 days after infection. Conclusions Rhesus macaques can be infected with H5N1 virus by endotracheal intubation, and it is proved to be a good method to obtain a more sensitive animal model of H5N1 virus infection. The experimental results will lay the foundation for further studies of pathogenetic mechanism of H5N1 infection.关键词
高致病性禽流感病毒H5N1/恒河猴/病毒血症/气管插管Key words
H5N1 virus/ Rhesus macaques/ viremia/ endotracheal intubation分类
医药卫生引用本文复制引用
吕琦,邓巍,鲍琳琳,许黎黎,李枫棣,陈霆,占玲俊,秦川..气管插管法接种H5N1病毒感染恒河猴及系统组织病毒检测[J].中国比较医学杂志,2011,21(12):56-60,5.基金项目
科技重大专项——艾滋病和病毒性肝炎等重大传染病防治:2009ZX10004-402 （）
科技重大专项——实验室生物安全保障技术平台的建立:2009ZX10004-503 （）
卫生部行业基金——实验动物和人类疾病动物模型资源扩展:200802036. （）
