中国比较医学杂志2012，Vol.22Issue(1)：62-65,4.DOI:10.3969/j.issn.1671.7856.2012.001.014
慢性间断性缺氧伴二氧化碳潴留小鼠模型的建立
A Mice Mode of Chronic Intermittent Hypoxia with Carbon Dioxide Retention
摘要
Abstract
Objective To establish a chronic intermittent hypoxia with carbon dioxide retention (CIH-CR)model in mice. Methods 22 male Kun Ming mice were divided into the normal control (NC) group and CIH-CR group. The mice of CIH-CR group suffered 8 hours intermittent hypoxia everyday for 4 weeks. The concentration of O2 and CO2 in the cabin was detected by oxygen analyzer and Carbon dioxide detector, meanwhile, the blood oxygen saturation were detected by blood oximeter. At the end of the experiment, weight of right and left ventricles was measured. The general pathological changes of myocardium, pulmonary, kidney and brain were observed by HE staining. Result The concentration of O2 and CO2 in the cabin and the SO2in the empennage of mouse accord with the cyclical change process. Compared with the NC group, the right ventricular hypertrophy were increased in the CIH-CR groups ( P < 0. 01 ). The tissue of myocardium, pulmonary, kidney and brain were showen obvious hypoxic change in CIH-CR group. Conclusion A CIH-CR animal model were be established successfully.关键词
慢性间断性缺氧/二氧化碳潴留/睡眠呼吸暂停综合征/模型,动物Key words
Chronic intermittent hypoxia/ Carbon dioxide retention/ Sleep apnea syndrome/ Model,animal分类
医药卫生引用本文复制引用
刘海林,张子彦,郭云云,李景春,宋永斌,徐江涛..慢性间断性缺氧伴二氧化碳潴留小鼠模型的建立[J].中国比较医学杂志,2012,22(1):62-65,4.基金项目
兰州军区医药卫生科研项目(LXH-2009024). （LXH-2009024）
