中国实用妇科与产科杂志2012，Vol.28Issue(1)：41-43,3.
组蛋白去乙酰化酶抑制剂对宫颈癌细胞的放射增敏作用
Radiosensitizing activity of SAHA on cervical cancer cells
徐赛 1邢军 1王慧1
作者信息
- 1. 河北联合大学医学院,河北唐山063000
- 折叠
摘要
Abstract
Objective To observe the radiosensitivity effect of histone deacetylase inhibitor SAHA on human cervical cancer SiHa cells. Methods MTT assay was used to measure the proliferation inhibition of SAHA on SiHa cells from October 2009 to June 2010 in Medical College of Hebei United University. The radiosensitivity effects of SAHA on SiHa cells with the concentration of 20% of IC50 were evaluated by the clonogenic assay. The software Sigma Plot 2000 Demo and the multi-target click model S = 1 - (1 - eD0/D) n was used to fit cell survival curve, determining radiation-related parameters and sensitive enhancement ratio (SER) to evaluate of sensitizing effect. Results MTT results showed that toxicity of SAHA on SiHa cell was in a dose and time dependent manner. The ICSO of the proliferation inhibition of SAHA on SiHa cells was 4. 80μmol/L. Cloning experimental results showed that cell survival rate was lower after treatment with the combination of SAHA (20% of IC50) and radiation than the cell survival rate with radiation alone. Both(s) the average lethal dose (DO) was 2. 329 and 1. 213 respectively, quasi-threshold dose (Dq) was 1. 721 and 0. 823 respectively. The sensitivity enhancement ratio (SER) was 1. 92. Conclusion SAHA has a good cytotoxicity and radiosensitization on SiHa cell.关键词
宫颈肿瘤/SiHa细胞/SAHA/放射增敏Key words
cervical cancer/ SiHa cells/ SAHA/ radiosensitization分类
医药卫生引用本文复制引用
徐赛,邢军,王慧..组蛋白去乙酰化酶抑制剂对宫颈癌细胞的放射增敏作用[J].中国实用妇科与产科杂志,2012,28(1):41-43,3.
