中国动物传染病学报2011，Vol.19Issue(4)：7-12,6.
非洲猪瘟病毒环介导恒温扩增快速检测技术的建立及应用
DEVELOPMENT OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION （LAMP） FOR RAPID DETECTION OF AFRICAN SWINE FEVER VIRUS
摘要
Abstract
A loop-mediated isothermal amplification （LAMP） rapid assay was developed for detecting African swine fever virus （ASFV） based on the vp72 gene. This method was compared to the PCR which was recommended by OIE. Seventeen genomic DNAs of ASFV isolates from African swine fever reference laboratory, 50 field pig samples and 30 field tick samples from China were detected by LAMP, respectively. Seventeen genomic DNAs of ASFV isolates could be detected successfully, but all of the field samples were negative. The result revealed that this method had a good specificity and sensitivity, which could be used in rapid diagnosis, prevention and control of African swine fever.关键词
环介导恒温扩增技术/聚合酶链式反应/非洲猪瘟/诊断Key words
Loop-mediated isothermal amplification （LAMP）/PCR/African swine fever（ASFV）/diagnosis分类
农业科技引用本文复制引用
杨吉飞,杜鹏飞,罗建勋,殷宏,关贵全,刘志杰,汪月凤,李有全,马米玲,刘爱红,任巧云,苟惠天..非洲猪瘟病毒环介导恒温扩增快速检测技术的建立及应用[J].中国动物传染病学报,2011,19(4):7-12,6.基金项目
“948”项目（2010-S04) （）
ASFRISK（211691) （）
“973”项目（2010CB530206) （）
国家自然科学基金项目（30800820、30972182、31072130、31001061) （）
甘肃省重点项目（N1002NKDA035、0801NKDA033) （）
农业部肉牛牦牛产业体系项目（CARS-38) （）
科技部合作专项欧盟,欧盟EPIZONE（FOOD-CT-2006-016236) （）
ARBOZOONET（211757) （）
PIROVAC（KBBE-3-245145)资助 （）
