中国动物传染病学报2011，Vol.19Issue(4)：19-24,6.
一株鹅细小病毒全基因特征分析
GENOMIC CHARACTERIZATIONS OF A PARVOVIRUS ISOLATED FROM GOOSE
摘要
Abstract
According to the genomic sequences deposited in GenBank, the complete genomic sequence of a parvovirus isolated from goose （GoPV） was amplified by PCR, and the all target fragments were cloned and sequenced. The results demonstrated that the inverted terminal repeat （ITR） at the 5＇ and 3＇ end of the genome was 444 nucleotides （nt）, and the full-genome contains two major open reading frames （ORF）. The left ORF containing 1884nts in length, from the position 537nt to 2420nt, encodes for non- structure proteins NS1 and NS2. The NS2 contains 1356nts in length, from the position 1065nt to 2420nt. Meanwhile, the right ORF encodes for viral structural proteins, VP1, VP2 and VP3. The VP1, VP2 and VP3 start from position 2439nt, 2874nt and 3033nt, respectively, and all end at the same C-terminal position 4637nt. There was also a Poly （A） tail before the right ITR. The nucleotide sequences of GoPV genome showed the highest homology （98.6%） with VG32/1（GenBank accession number EU583392）. This is the first report about the complete nucleotide sequence of GoPV in the mainland of China.关键词
鹅细小病毒/全基因/分子特征Key words
Goose parvovirus/full-genome/molecular characterization分类
农业科技引用本文复制引用
万春和,朱海侠,黄瑜,彭春香,程龙飞,施少华,傅光华,陈红梅..一株鹅细小病毒全基因特征分析[J].中国动物传染病学报,2011,19(4):19-24,6.基金项目
公益性行业（农业)科研专项经费项目（201003012) （）
