中国动物传染病学报2011，Vol.19Issue(4)：48-54,7.
重组日本血吸虫抱雌沟蛋白诱导的免疫机制研究
THE STUDY OF THE MECHANISM INDUCED BY RECOMBINANT SCHISTOSOMA JAPONICUM GYNECOPHORAL CANAL PROTEIN
摘要
Abstract
For investigating the immune mechanism induced by the vaccine candidate Schistosoma japonicum gynecophoral canal protein（SjGCP）, BALB/c mice were vaccinated with the recombinant protein SjGCP mixed with three different adjuvants FCA, ISA 206 and ISA 70M, respectively. The percentage of CD4＋ and CD8＋ T lymphocyte subsets and the cytokines IL-2, IL-4, γ-IFN and IL-10 in splenic lymphocytes from mice post three immunizations were analyzed by flow cytometry, and the serum of each groups were collected for detecting specific antibodies IgG and IgG1 by ELISA. The results showed that the percentage of CD4＋ T cells in GCP-70M group and CDS＋ T cells in GCP-FCA and GCP-206 groups decreased significantly post three immunizations, comparing to the PBS control group, while the percent of CD3＋CD8-IL-4＋ and CD3＋CD8-IL-10＋ cells in groups of GCP-FCA and GCP-206 increased. The ELISA results showed that the levels of the specific antibodies IgG 1 subtype in the sera of GCP-FCA, GCP-206 and GCP-70M groups were significantly higher than that of control groups. These results accumulated the valuable data for understanding the protection mechanism induced by recombinant protein SjGCP.关键词
日本血吸虫抱雌沟蛋白/免疫机制/淋巴细胞亚群/细胞因子Key words
Schistosomajaponicum/gynecophoral canal protein/immune mechanism/lymphocyte subsets/cytokine分类
农业科技引用本文复制引用
李东,朱珠,石耀军,陆柯,彭金彪,洪炀,张旻,韩彦辉..重组日本血吸虫抱雌沟蛋白诱导的免疫机制研究[J].中国动物传染病学报,2011,19(4):48-54,7.基金项目
国家863计划（2006AAl0A207) （）
