中国动物传染病学报2011，Vol.19Issue(4)：67-70,4.
多房棘球绦虫-泡球蚴的体外培养研究
STUDY ON IN VITRO CULTIVATION FOR PROTOSCOLECES OF THE ECHINOCOCCUS MULTILOCULARIS
摘要
Abstract
To option cultural conditions for protoscoleces of Echinococcus and to observe their growth and development granulosus in vitro. Protoscoleces（PSC） were collected from hydatid cysts of E. multilocularis and the PSC were cultured in three media, RPMI-1640, MEM and M199 with fetal calf serum （FCS）. Survival rate of the PSC cultured for 9 days was respectively 90.10% in RPMI-1640 with FCS, 50.25% in MEM with FCS and 22.03% in M199 with FCS. The rate of encystation was respectively 57.12% in RPMI-1640 with FCS, 63.15% in MEM with FCS, 48.17% in M199 with FCS. The rate extraversion of Scolex was respectively 98.28% in RPMI-1640 with FCS, 88.65% in MEM with FCS, 75.50% in M199 with FCS. The most PSC were developed to cyst formotion in the early stage, some of the PSC developed to adult worm, and later change to cysts. Medium RPMI-1640 with FCS is more suitable medium for culturing PSC to hydatid cysts in virto.关键词
多房棘球绦虫/泡球蚴/体外培养Key words
Echinococcus multilocularis/protoscolex/in vitro cultivation分类
农业科技引用本文复制引用
米晓云,古努尔·吐尔逊,张壮志,石保新,金映红,张旭,赵莉,张文宝..多房棘球绦虫-泡球蚴的体外培养研究[J].中国动物传染病学报,2011,19(4):67-70,4.基金项目
家畜疫病病原生物学国家重点实验室项目资助 （）
