中国动物传染病学报2011，Vol.19Issue(4)：71-75,5.
三种鉴别诊断PRRSV美洲型经典毒株和变异株的方法比较
A COMPARATIVE STUDY OF THREE DIAGNOSTIC METHODS FOR DIFFERENTIAL DETECTION OF CLASSICAL PRRSV AND PRRSV VARIANT
摘要
Abstract
Three diagnostic method including the virus isolation and identification （VI）, direct immunofluorescence assay （DFA） and reverse transcriptase polymerase chain reaction （RT-PCR） were employed to detect 99 specimens , parallelly. The results showed that the coincidence between DFA and VI, DFA and RT-PCR were 92.5% and 92.2%, respectively. RT-PCR was the highest sensitivity and specificity, which could be used as the first choice for laboratory differential diagnosis. DFA was high specificity and fast, especially useful for PRRS＇s clinic detecting. The virus isolation＇s procedure was complicated, consumed long time, had a low sensitivity and positive separation needed other methods to identification. Therefore virus isolation was not a suitable test for distinguish detection.关键词
猪繁殖与呼吸综合征病毒美洲型经典毒株/猪繁殖与呼吸综合征病毒变异株/RT-PCR/DFA免疫荧光诊断试剂盒/病毒分离Key words
classical Porcine reproductive and respiratory syndrome （PRRSV）/variant PRRSV/RT-PCR/direct immunofluorescenceassay （DFA）/virus isolation （VI）分类
农业科技引用本文复制引用
陈仕龙,郑敏,陈少莺,江斌,林锋强,朱小丽,王劭,程晓霞,黄梅清,李兆龙..三种鉴别诊断PRRSV美洲型经典毒株和变异株的方法比较[J].中国动物传染病学报,2011,19(4):71-75,5.基金项目
福建省科技计划项目（2008N0027)和福建省农科院创新团队项目（STIF-Y02) （）
