中国动物传染病学报2011，Vol.19Issue(5)：32-38,7.
福建省H9N2亚型禽流感病毒HA和NA基因的进化分析
PHYLOGENETIC ANALYSIS OF HAEMAGGLUTININ AND NEURAMINIDASE GENES OF H9N2 INFLUENZA VIRUSES ISOLATEDFROM FUJIAN PROVINCE
摘要
Abstract
Haemagglutinin（HA） and neuraminidase（NA） genes of two H9N2 subtype viruses（FZ-04 and FZ-11） isolated from different broiler flocks in Fujian in 2011 were amplified and sequenced in order to determine their genetic relationship with other Avian influenza virus strains that were either isolated from the same area during 2000-2011 or deposited in GenBank.The nucleotide sequences of haemagglutinin genes of both strains had the highest identity with CK/FJ/G9/09,indicating these strains belonged to the same major genetic CK/BJ/1/94 like sublineage.The FZ-04 and FZ-11 strains had-PSRSSR/GL-sequence at HA proteolytic cleavage sites,a characteristic of low pathogenic Avian influenza virus（LPAIV）.The neuraminidase gene of FZ-04 and FZ-11 strains encoded 469 amino acid sequences and had no nucleotides deletion.Phylogenetic analysis of NA genes showed that both strains had the highest homology with CK/FJ/10954/05 and belonged to genetic CK/HK/G9/97 like sublineage.On the basis of phylogenetic analysis of HA and NA genes,we concluded that H9N2 subtype Influenza viruses circulating in chicken flocks in Fujian during 2000-2011 might have a stable evolution and common origin.关键词
禽流感病毒/H9N2亚型/HA基因/NA基因/进化分析Key words
Avian influenza virus/H9N2 subtype/haemagglutinin/neuraminidase/phylogenetic analysis分类
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朱春华,江斌,林甦,刘斌琼,陈珍,程龙飞,黄瑜..福建省H9N2亚型禽流感病毒HA和NA基因的进化分析[J].中国动物传染病学报,2011,19(5):32-38,7.基金项目
福建省重点项目（2009N4001) （）
院青年创新基金（2009QB-15) （）
