中国动物传染病学报2011，Vol.19Issue(5)：50-56,7.
日本血吸虫Ⅴ型胶原蛋白α1链的原核表达分析
EXPRESSION OF SCHISTOSOMA JAPONICUM TYPE V COLLAGEN A1 CHAIN IN E.COLI BL21
杨云霞 1石艳丽 1刘萍萍 1刘金明 1金亚美1
作者信息
- 1. 中国农业科学院上海兽医研究所,上海200241
- 折叠
摘要
Abstract
To analyze expression of Schistosoma japonicum type V collagen a1 chain in E.coli BL21（DE3）,a prokaryotic expression vector pET28-SjColV（a1） was constructed.rSjColV（a1） was expressed in E.coli BL21（DE3） with induction of IPTG and confirmed in SDS-PAGE and Western blot.The results showed that SjColV（a1） was efficiently expressed in BL21（DE3）,but the products were unstable.In this study we analyzed conditions for prokaryotic expression of SjCol V（a1） and proposed future studies for the application of SjColV（a1） protein.关键词
胶原蛋白/rSjColV（a1）/原核表达Key words
collagen/rSjColV（a1）/prokaryotic expression分类
农业科技引用本文复制引用
杨云霞,石艳丽,刘萍萍,刘金明,金亚美..日本血吸虫Ⅴ型胶原蛋白α1链的原核表达分析[J].中国动物传染病学报,2011,19(5):50-56,7.
