中国动物传染病学报2011，Vol.19Issue(6)：1-6,6.
猪繁殖和呼吸综合征病毒HuN4株nsp9、nsp10、nsp11对毒力影响的研究
EFFECTS OF NONSTRUCTURAL PROTEINS 9, 10 AND 11 ON THE VIRULENCE OF HIGHLY PATHOGENIC PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS
摘要
Abstract
In order to investigate if nonstructural proteins were necessary for the virulence of the highly pathogenic Porcine reproductive and respiratory syndrome virus （HP-PRRSV）, we generated three full length infectious cDNA clones by using reverse genetic manipulation, in which the three coding regions for nsp9, nspl0 and nspll of the attenuated live PRRSV HuN4-Fll2 were respectively substituted by the corresponding regions of HP-PRRSV HuN4-F5. The three recombinant viruses （rHuN4-F112- nsp9, rHuN4-F112-nsp 10 and rHuN4-F112-nsp11） were rescued and confirmed in indirect immunofluorescence assay and genomic sequencing. The results from growth studies on MARC-145 cells demonstrated that rHuN4-Fll2-nsp9 and rHuN4-F112-nsp10 showed significantly higher titers than rHuN4-F 112-nsp 11 and rHuN4-F 112 at the first 24 hours and then turned to no difference. After inoculation into PRRSV-negative piglets, no differences in body temperature, clinical signs, viremia and lesions were observed between recombinant viruses rHuN4-F 112-nsp9, rHuN4-F 112-nsp 10, rHuN4-F 112-nsp 11 and rHuN4-F 112. Therefore, we concluded that nonstructural proteins nsp9, nsp 10 and nsp 11 were not individually related to the virulence of HPPRRSV.关键词
高致病性猪繁殖和呼吸综合征病毒/nsp9/nsp10/nsp11Key words
Highly pathogenic Porcine reproductive and respiratory syndrome virus （HP-PRRSV）/nsp9/nspl0/nsp 11分类
农业科技引用本文复制引用
姜一峰,周艳君,王亚欣,朱建平,虞凌雪,徐彦召,童武,童光志..猪繁殖和呼吸综合征病毒HuN4株nsp9、nsp10、nsp11对毒力影响的研究[J].中国动物传染病学报,2011,19(6):1-6,6.基金项目
NSFC-广联合基金项目（U0931003) （）
上海市科技人才计划项目（09XD1405400) （）
国际合作项目（2010DFB33920) （）
国家生猪现代产业技术体系建设项目（NYCYTX-009) （）
中央科研院所公益性基础科研业务费项目（2011JB03) （）
