中国兽医科学2011，Vol.41Issue(8)：789-793,5.
山羊关节炎-脑炎病毒陕西分离株全基因组的克隆和序列分析
Cloning and sequence analysis of the whole genome of caprine arthritis-encephalitis virus Shaanxi strain
摘要
Abstract
Five pairs of primers were designed according to the nucleotide sequences of caprine arthritis-encephalitis virus（CAEV） deposited in GenBank,and 5 fragments covering the whole viral genome were obtained and sequenced from infected cells. The whole genome of CAEV Shaanxi strain consists of 9 065 nucleotides,including two LTRs, three structural gene（gag, pol and env gene）, and three regulatory gene （tat, vif and rev gene）. The accession number of CAEV Shaanxi strain in GenBank is GU120138. Comparative analysis with the genomic sequences of CAEV Gansu and CAEV CO strain revealed that CAEV Shaanxi shared 99.6% identity with CAEV Gansu and 92.4% with CAEV CO. Phylogenetic analysis based on complete genome and gag gene, revealed that the CAEV Shaanxi strain had closer relationship with CAEV Gansu strain and CAEV CO strain in comparison with the Italian and Norway strains, and also showed that CAEV Shaanxi strain belonged to SRLV subtype B2.关键词
山羊关节炎-脑炎病毒/基因克隆/基因组解析/进化分析Key words
caprine arthritis-encephalitis virus/gene cloning/genome anatomy/phylogenetic analyses分类
农业科技引用本文复制引用
黄金海,孙跃辉,庄世文,肖华志,张丽霞..山羊关节炎-脑炎病毒陕西分离株全基因组的克隆和序列分析[J].中国兽医科学,2011,41(8):789-793,5.基金项目
国家自然科学基金项目（30771613) （）
