中国兽医科学2011，Vol.41Issue(8)：799-805,7.
沙门氏菌invH基因的重组表达与免疫保护效果研究
Expression and immunogenicity study of invH gene of Salmonella typhimurium
摘要
Abstract
The objective of the present study was to obtain subunit vaccine of invH gene of Salmonella typhimurium and to evaluate its immunogenicity. Gene of invasionH（invH） from S. typhimurium DT104 amplified by polymerase chain reaction（PCR） was cloned into pGEX-4T-3 getting the recombinant pGEX/ invH,with the invH coexpressed with GST. Mice were injected with three different doses of fusion protein with oil emulsion adjuvant,and two groups were injected with inactivated vaccine and PBS respectively, to evaluate the immune efficacy. The bioactivity of antisera was detected by adherence inhibition assay. Western-blotting analysis indicated that the fusion protein was 44 ku in molecular weight and had immunological activity. We immunized with different doses of the fusion protein invH to mice, challenged with 100-fold LD50 amount of S. typhimurium DT104（4.23〈 107 CFU） ,and found that the survive rate of protein vaccine groups was more than 60% （P〈0. 01）, which are lower than that of inactivated vaccine, but significantly higher than that of control group. And the three groups of protein vaccine all raised levels of mice serum IL-4 and IFN-γ （P〈0.05）. The antiserum of expressed protein can inhibit adherence activity of S. typhimurium DT104 to cell surface by adherence inhibition assay. In conclusion,invH has certain immunogenicity and can offer partial protection against high dose challenge, which provides a theoretical basis for the prevention of S. typhimurium.关键词
沙门氏菌/invH基因/融合表达/免疫保护/黏附抑制Key words
Salmonella/invH gene/fusion expression/immune protection/adhesion inhibition分类
农业科技引用本文复制引用
王小龙,张伟华,牛钟相..沙门氏菌invH基因的重组表达与免疫保护效果研究[J].中国兽医科学,2011,41(8):799-805,7.基金项目
国家“十一五”科技支撑计划项目（2006BAD06A11) （）
