中国兽医科学2011，Vol.41Issue(8)：817-820,4.
猪传染性胃肠炎病毒S蛋白基因的克隆与表达
Cloning and expression of S protein gene of swine transmissible gastroenteritis virus
摘要
Abstract
According to the complete genome sequence of swine transmissible gastroenteritis virus （TGEV） TS strain in GenBank,gene of 980 bp in size for S protein with loci A and D was amplified by RT- PCR,cloned into the Simple-T vector. The constructed plasmid Simple-T-S was confirmed by restriction enzyme digestion and sequencing. The gene were then cloned into the expression vector pET-28a（＋）,and transfered into Rossetta（DE3） competent cells. The protein was expressed with the induction of IPTG and analyzed by SDS-PAGE and Western-blot. In result,the expressed product was about 33.7 ku in molecular mass,mainly in the form of inclusion bodies. Western-blot analysis showed that the expressed S protein could react with the rabbit anti-TGEV-positive serum.关键词
猪传染性胃肠炎病毒/S基因/原核表达Key words
swine transmissible gastroenteritis virus（TGEV）/S gene/prokaryotic expression分类
农业科技引用本文复制引用
刘媛,左玉柱,范京惠..猪传染性胃肠炎病毒S蛋白基因的克隆与表达[J].中国兽医科学,2011,41(8):817-820,4.基金项目
河北农业大学校长基金项目 （）
