中国兽医科学2011，Vol.41Issue(9)：881-885,5.
羊源巴贝斯虫未定种甘肃敦煌株的发现
Discovery of an ovine Babesia sp. strain from sheep in Dunhuang City of Gansu Province
摘要
Abstract
Mixture of field-collected blood from 15 sheep in Dunhuang City was injected into a spleenectomized sheep.A large number of Babesia were present in the blood smeared on day 10 post-inoculation.The major morphological forms included paired piriform,single piriform,ring form and oval form.On day 12 post-inoculation,the body temperature and parasitemia of sheep reached 41.1 ℃ and 0.125% respectively and then decending.Genomic DNA was extracted from whole blood sterilely collected from the infected sheep.A 1 700 bp fragment was amplified by PCR using universal primers based on the 18 S rRNA gene.Then the target fragment was cloned and sequenced（GenBank accession number：HQ730762）.Multiple sequence alignments were performed with those of other Babesia in GenBank.It shared the highest similarity with Babesia sp.Xinjiang,which was up to 99.8%.The present study discovered another epidemic focus of Babesia sp.Xinjiang in Dunhuang City of Gansu Province.关键词
绵羊血液/巴贝斯虫/染虫率/18S/rRNA/聚合酶链反应Key words
sheep blood/Babesia/parasitemia/18 S rRNA/PCR分类
农业科技引用本文复制引用
马米玲,王朝文,刘志杰,罗建勋,殷宏,关贵全,苟惠天,杨吉飞,万玉林,李有全,刘爱红,任巧云,陈泽..羊源巴贝斯虫未定种甘肃敦煌株的发现[J].中国兽医科学,2011,41(9):881-885,5.基金项目
国家自然科学基金资助项目（30800820 30972182 31072130 31001061) （）
甘肃省重点项目（1002NKDA035 0801NKDA033) （）
欧盟项目EPIZONE（FOOD-CT-2006-016236)、ASFRISK（211691)、ARBOZOONET（211757)和PIROVAC（KBBE-3-245145) （）
