中国兽医科学2011，Vol.41Issue(9)：901-906,6.
表达猪瘟病毒E2蛋白重组减毒鼠伤寒沙门氏菌活载体疫苗株的构建及动物免疫试验
Construction and immunogenicity of the recombinant attenuated Salmonella typhimuriumlive vector vaccine strain expressing CSFV E2 antigen
摘要
Abstract
In order to construct a recombinant attenuated Salmonella typhimurium oral live vector strain expressing E2 antigen of classical swine fever virus（CSFV）,the CSFV E2 gene was subcloned and inserted into the expression vector pYA3341.The recombinant plasmid pYA3341-E2 was finally electro-transformed into delta cya,delta crp,delta asd attenuated S.typhimurium vaccine strain X4550 to construct the live vaccine strain X4550（pYA3341-E2）.The recombinant X4550（pYA3341-E2） strain was identified from E2 protein expression,stability,growth curve,safety in vitro and immunity evaluation by animal experiment in swine.SDS-PAGE and Western-blotting analyses showed that the E2 protein expressed in the S.typhimurium X4550（pYA3341-E2） could be recognized by the positive serum of anti-CSFV.The recombinant strain could stably propagate at noruishment selection pressure in vitro.The recombinant strain X4550（pYA3341-E2） could colonize and persist stably in mesenteric lymph nodes and spleens of BALB/c mice with its safety and reliability verified by mouse experiment.The recombinant strain X4550（pYA3341-E2） was immunized orally to swine and the E2 protein serum antibody could be detected.Lymphocyte proliferative response results indicated that the recombinant strain can also induced an obvious cellular immune response in swine.Results showed that a recombinant oral live vaccine S.typhimurium strain X4550（pYA3341-E2） which can express CSFV E2 protein stably was constructed successfully,and this live vaccine could be a basis to oral genetic engineering vaccine of CSF.关键词
猪瘟病毒/E2蛋白/减毒鼠伤寒沙门氏菌/疫苗Key words
classical swine fever virus/E2 protein/attenuated Salmonella typhimurium/vaccine分类
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许信刚,王丹,童德文..表达猪瘟病毒E2蛋白重组减毒鼠伤寒沙门氏菌活载体疫苗株的构建及动物免疫试验[J].中国兽医科学,2011,41(9):901-906,6.基金项目
国家大学生创新性实验计划项目（2010) （）
西北农林科技大学青年学术骨干项目（E111020901) （）
陕西省农业科技创新项目（2010NKC-06) （）
