中国兽医科学2011，Vol.41Issue(9)：941-944,4.
山羊背根神经节神经细胞的原代培养及鉴定
In vitrocultivation and identification of goat dorsal root ganglia cells
摘要
Abstract
The aim of the present study was to get the goat dorsal root ganglia（DRG） cells from the goat embryo.The DRG was isolated from goat embryo of three months old,and then were cultured with tissue culture method using both serum and serum-free medium.The growth of the DRG cells was observed in vitro under the effect of non-neuro cell inhibitor.NSE,the neuro cell surface markers was detected with immunofluorescence and RT-PCR.The cells were immigrated from the tissue on hour 48 after incubation,and the neurite was visible.After the effect of cytarabine,neurons and non-neuro cell were demixing,and neurons was identified in the over layer by immunofluorescence.The neurites were distinguished extended and connected into nets as time went on.Cells were fully developed on day 6 to 10,and then began aging;and it was observed that 32 days were the longest life span.The DRG cells were successfully isolated from the goat embryo and cultured in vitro.关键词
神经元/体外培养/背根神经节/山羊Key words
neuron/culture in vitro/dorsal root ganglia/goat分类
生物科学引用本文复制引用
吴卫妮,任妮,王志豪,范洁,徐永平..山羊背根神经节神经细胞的原代培养及鉴定[J].中国兽医科学,2011,41(9):941-944,4.基金项目
陕西省农业推广专项（14220201) （）
