中国兽医科学2011，Vol.41Issue(10)：991-994,4.
建立在PCR-RFLP方法基础上的羊梨形虫的种类鉴定
Detection and differentiation of the ovine piroplasm species based on the PCR-RFLP method
摘要
Abstract
Ovine piroplasmosis is an emerging disease worldwide,with multiple species of Babesia ssp. and Theileria spp.now being recognized to infect sheep and goats.Five piroplasms,including B.motasi,B.sp.,T.luwenshuni,T.uilenbergi and T.ovis,have been reported in China,and also caused the great economic losses to stock raising.To develop a taxonomic tool for piroplasm species infective to the sheep and goats,the 18S rRNA genes from all species were amplified using PCR,and digested by Cla Ⅰ,Sau96Ⅰ and Mbo Ⅱ.Results showed that B.motasi,B.sp.,T.luwenshuni,T.uilenbergi and T.ovis can be effectively discriminated based on the restriction fragment length polymorphism（RFLP）.The present study provided a novel assay for molecular identification and taxonomy of piroplasm.关键词
梨形虫/泰勒虫/巴贝斯虫/限制性片段多态性Key words
piroplasm/Theileria/Babesia/PCR-RFLP分类
农业科技引用本文复制引用
徐宗可,关贵全,刘爱红,马米玲,任巧云,殷宏,罗建勋..建立在PCR-RFLP方法基础上的羊梨形虫的种类鉴定[J].中国兽医科学,2011,41(10):991-994,4.基金项目
国家自然科学基金项目（30972182) （）
