中国兽医科学2011，Vol.41Issue(10)：1001-1004,4.
秀丽隐杆线虫的培养与保存研究
Study on the culture and preservation of Caenorhabditis elegans
摘要
Abstract
Growth effect of Caenorhabditis elegans on four different medium including OP50-NGM,JM109-NGM,OP50-LB and JM109-LB were evaluated and compared with each other.The preserving effects of C.elegans in three preserving solutions,with different proportional glycerol were evaluated under the condition of liquid nitrogen-80℃ and-20℃,respectively.Results showed that C.elegans grew better on the NGM medium covered with E.coli OP50,than other three mediums.For preserving solution,C.elegans had a survival rate of 78% in LB medium containing 50mL/L glycerol at-80℃ and it had a survival rate of 52% in PBS at the same condition as LB medium,while in water with 100mL/L glycerol,the survival rate was only 8%.Both adults and larvae can survive in liquid nitrogen,but only the larvae can survive at-80℃and-20℃.In conclusion,C.elegans grew well on NGM medium covered with E.coli OP50,and the optimum preserving condition was LB medium with 50mL/L glycerol at-80℃.关键词
秀丽隐杆线虫/培养/保存Key words
Caenorhabditis elegans/culture/preservation分类
农业科技引用本文复制引用
李有全,关贵全,彭欲率,何海宁,罗建勋,殷宏..秀丽隐杆线虫的培养与保存研究[J].中国兽医科学,2011,41(10):1001-1004,4.基金项目
国家肉牛产业技术体系项目（CARS-38) （）
国家自然科学基金项目（30800820) （）
欧盟项目EPIZONE（FOOD-CT-2006-016236) （）
