中国兽医科学2011，Vol.41Issue(11)：1144-1149,6.
猪瘟病毒复合多表位抗原基因的克隆表达及其免疫学特性
Cloning,expression and immunological characteristics of compound multi-epitope gene of classical swine fever virus
摘要
Abstract
To develop multi-epitope vaccine against classical swine fever, a multi-epitope gene BT23 was optimized and artificially synthesized according to the epitope to sequence of classical swine fever virus（CSFV） in GenBank and literatures,and was then cloned into the pMD18-T vector. After BamHⅠ and EcoRⅠ double digestion,BT23 gene was recovered and subcloned into the pGEX-6P-1 vector to form a recombinant plasmid pGEX-BT23. And then the BT23 gene in pGEX-BT23 was expressed in Escherichia coli BL21（DE3） competent cells. The highest expressed fusion protein was 30% of total bacterial protein after being induced with IPTG at 0.9mmol/L for 8h in a inclusion body manner with molecular weight of 36ku as certified by SDS-PAGE. Western-blot analysis showed that the expressed product had good reactinogenicity,which specially reacted with CSFV positive sera. Moreover,the fusion protein could induce an efficient immune protection against HCLV infection,which provide foundations for the preparation of the CSFV serological diagnostic reagents and for the development of multi-epitope vaccine.关键词
猪瘟病毒/复合多表位抗原基因/融合表达/活性分析Key words
classical swine fever virus/compound multi-epitope gene/fusion expression/activity analysis分类
农业科技引用本文复制引用
侯相民,田宏,吴锦艳,陈妍,尚佑军,刘湘涛..猪瘟病毒复合多表位抗原基因的克隆表达及其免疫学特性[J].中国兽医科学,2011,41(11):1144-1149,6.基金项目
国家高技术研究发展计划（863)项目（2006AA10A204) （）
