中国兽医科学2011，Vol.41Issue(11)：1154-1159,6.
结核分枝杆菌三价DNA疫苗的体液免疫与细胞免疫应答
Humoral and cellular immune responses elicited by the trivalent DNA vaccine against Mycobacterium tuberculosis
摘要
Abstract
In order to evaluate the immune response in vivo induced by the DNA vaccine based on Mycobacterium tuberculosis Ag85B,MPT83 and MPT64 antigen in cow, three plasmids were extracted by anion exchange chromatography method and three-month-old Holstein cows were immunized with the eukaryotic expression vector PJW4303 and dime-thyldioctyldeeyl ammonium bromide. Enzyme-linked immunosorbent assay（ELISA） was used to detect anti-Ag85B,MPT83 and MPT64 antibody titers in sera of the vaccinated cow,and double antibody sandwich ELISA was used to quantitatively determine the concentration of IFN-γ. Results showed that the purified DNA vaccines met the require of animal immune test. The specific antibody titers of cattle were negative before the pre-immunization,and MPT83,Ag85B and MPT64 can induce to the highest antibody titers on day 30 after third immunization with average titers of 1∶3200、1∶2560 and 1∶2880. Antigen-specific IFN-γ level of MPT83,Ag85B,MPT64 and PBS were（630.25±94.45）pg/mL,（341.65±76.75）pg/mL,（51.38±9.80）pg/mL and（6.48±20.88）pg/mL, respectively. The negative control group（PBS） showed significant difference（P0.01） with the three antigen groups in each month.In conclusion,the trivalent DNA vaccines can simulate humoral and cellular immune responses simultaneously,which suggested that the trivalent DNA vaccines might be able to enhance protective response of cow against tuberculosis.关键词
结核分枝杆菌/阴离子交换色谱/DNA疫苗/免疫应答Key words
Mycobacterium tuberculosis/anion exchange chromatography/DNA vaccine/immune response分类
农业科技引用本文复制引用
张云峰,刘福元,蔡宏,呼西旦,宋振忠,徐雪萍..结核分枝杆菌三价DNA疫苗的体液免疫与细胞免疫应答[J].中国兽医科学,2011,41(11):1154-1159,6.基金项目
新疆生产建设兵团科技支疆计划项目（2008ZJ06) （）
新疆农垦科学院青年基金项目（YQJ2009-04) （）
