中国兽医科学2011，Vol.41Issue(12)：1215-1222,8.
布鲁氏菌全基因组DNA芯片研制及比较基因组杂交方法的建立
Whole genome DNA microarray construction and comparative genomic analysis of Brucella
摘要
Abstract
To develop whole genome DNA microarray based on the genomic of Brucella,a total of 3 218 genes of Br.abortus 16M and Brucella9-941 were downloaded from GenBank for primers design.After several rounds of condition optimization and redesign of primers,PCR products of 3 212 genes were finally obtained,purified and printed onto glass slides to construct a microarray.The microarray includes a total of 6 912 probes,including double spots for each gene,and positive and negative control.Using Cy3/Cy5 double staining the genes of Brucella 16M as reference sample and genes of Br.abortus 9-941 test samples,the comparative genomic hybridization was developed.The microarray hybridization results were perfectly consistent with the theory result.In conclusion,the microarray can be used as a useful tool for comparative genomic analysis of Brucella.关键词
布鲁氏菌/DNA芯片/比较基因组学Key words
Brucella/whole genome DNA microarray/comparative genomics分类
农业科技引用本文复制引用
钟志军,付思美,王同坤,黄克和,陈泽良,彭广能,徐杰,于爽,王玉飞,周冬生,汪舟佳,杜昕颖,白耀霞,陈燕芬..布鲁氏菌全基因组DNA芯片研制及比较基因组杂交方法的建立[J].中国兽医科学,2011,41(12):1215-1222,8.基金项目
国家重点基础研究发展计划(973)项目(2009CB522602) （973）
国家自然科学基金项目(31000548) （31000548）
教育部"长江学者和创新团队发展规划"创新团队项目(IRT0848) （IRT0848）
四川农业大学双支计划项目(00270713) （00270713）
