中国兽医科学2011，Vol.41Issue(12)：1282-1287,6.
KiSS-1基因在黄牛精子发生中的表达
Expression of KiSS-1 gene in bovine testis spermatogenesis
摘要
Abstract
To explore the physiologic function of the kisspeptins in bovine spermatogenesis as well as provide reference to research reproductive physiology of livestock,kisspeptins-1（Kiss-1） expression characteristics in the testis of adult cattle were studied by using SABC immunohistochemical staining and image analysis software（Image Pro-Plus6.0）.In result,the KiSS-1 immunopositive production were found in the spermatogonia,primary spermatocytes,secondary spermatocytes,early spermatids and interstitial cells of the adult bovine testes,with the order of the total optical density of the positive cells（IOD） value as IODprimary spermatocytes IODinterstitial cells IODsecondary spermatocytes〉IODspermatogonia 〉IODearly spermatids（P〈0.05）.However,the production were not found in the late spermatids,sperm,sertoli cells and interstitial blood vessels of the adult cattle testes.The large KiSS-1 immunoreactivity production was seen around the periphery of some interstitial cells,while this phenomenon was not found in other positive cells periphery.The results suggested that KiSS-1 gene plays a very important role in the spermatogenesis of cattle testes,and may especially play very important regulative role in the spermatogonia mitosis and spermatocytes meiosis,and the testicular interstitial cells have the function of KiSS-1 secretion in the testes microenvironment.关键词
亲吻素-1/精子发生/睾丸/黄牛Key words
kisspeptins-1/spermatogenesis/testis/cattle分类
农业科技引用本文复制引用
邵宝平,王继卿,罗玉柱,王建林,陈建龙,柴尔青,徐元青..KiSS-1基因在黄牛精子发生中的表达[J].中国兽医科学,2011,41(12):1282-1287,6.基金项目
甘肃省重点实验室建设计划项目(085RTSA004) （085RTSA004）
教育部博士点-新教师基金项目(20090211120002) （20090211120002）
国家自然科学基金青年科学基金项目(31000190) （31000190）
