中国畜牧兽医2011，Vol.38Issue(11)：38-41,4.
水牛小腔卵泡分离方法的初步研究
Studies on Isolation of Buffalo Small Antral Follicles
摘要
Abstract
This article was conducted to study the factors of isolation buffalo small antral follicles,so that to establish the effective isolation method of buffalo small antral follicles. The results showed that the method of mechanical combine with enzyme harvested significantly more small antral follicles (5. 64) than mechanical method (3. 12,P<0. 05). The average number of small antral follicles harvested from ovary was increased as enhancing the digesting time of enzyme,and the groups of 12 min and 15 min were significantly higher than that of digesting for 5 and 10 min(P<0. 05) ,but when digesting time of enzyme was prolonged to 20 min, the theca of follicles was broken out that could not isolate the intact small antral follicles. Most small antral follicles harvested from ovary which surface had less than 2 mm follicles but no corpora luteum significantly higher than that of ovary which surface had corpora luteum but no follicles(7. 50 and 2. 25,P<0. 05). These results indicated that selecting the ovary which surface had less than 2 mm follicles but no corpora luteum and adopting the method of mechanical combine with enzyme to digest 12 to 15 min was fit for isolating the buffalo small antral follicles.关键词
水牛/小腔卵泡/分离Key words
buffalo/ small antral follicles/ isolation分类
农业科技引用本文复制引用
李翠玲,李楠,黄章虎,蒋建荣,陆凤花,石德顺..水牛小腔卵泡分离方法的初步研究[J].中国畜牧兽医,2011,38(11):38-41,4.基金项目
国家农业部转基因重大专项(2008ZX08007-003) （2008ZX08007-003）
国家自然科学基金项目(31072033). （31072033）
