中国畜牧兽医2011，Vol.38Issue(11)：57-60,4.
不同品种牛血清对3T3L1前脂肪细胞增殖与分化的影响
Effects of Different Species Bovine Sera on Proliferation and Differentiation in 3T3L1 Preadipocyte
摘要
Abstract
The purpose of this study was to know the effects of different species bovine sera on the proliferation and differentiation of 3T3-L1 preadipocytes. In order to provide the possible method of early selection for marbling, we use cell model in vitro culture to simulate the environment of bovine adipose tissue. Collected the bovine sera of different species from seventeen Qinchuan cattle and twenty-eight Qinchuan- Angus hybrid cattle. One part of sera were heat inactivated. The other were activated. The relatively cell number, which stands for proliferation of 3T3-L1 cells, was assayed by MTT colorimetry. Fat content and enzyme activities,which contain glycerol phosphate dehydrogenase (GPDH) and fatty acid synthetase (FAS), represented the differentiation. Oil red O staining was used to determine the fat content, and enzyme activities were determined by colorimetry after inducting adipogenesis. The results showed as follow. First, heat inactivated sera were quite significantly higher than activated ones on relatively cell number of 3T3-L1 cell at day 2 and 4,and it's the same results with fat content at day 2,4,6 and 8 (P<0. 01). Second,Qinchuan - Angus hybrid bovine sera were significantly higher than Qinchuan bovine sera on relatively cell number at day 2 and 4 in cell culture (P<0. 05). Qinchuan sera were significantly higher than hybrid sera on lipid content at day 8 (P<0. 05). However,there were no significant different at other days on cell number and lipid content (P>0. 05). Moreover,there were no significantly different between two species of cattle sera on GPDH and FAS activities in cell culture at day 8 (P>0. 05). Self-made bovine sera within heat inactivation promoted proliferation and differentiation of 3T3-L1 cell. Bovine sera is one of the factors that affect proliferation and differentiation of 3T3-L1 cell. Qinchuan- Angus hybrid bovine sera may be good for proliferation,wahile Qinchuan bovine sera may be good for differentiation. More studys are needed.关键词
品种/牛血清/增殖/分化/3T3-L1前脂肪细胞Key words
species/ bovine sera/ poliferationfdifferentiation/ 3T3-Ll preadipocyte分类
生物科学引用本文复制引用
冯丽萍,宋忠峰,施琼,李文波,罗晓瑜,曹兵海..不同品种牛血清对3T3L1前脂肪细胞增殖与分化的影响[J].中国畜牧兽医,2011,38(11):57-60,4.基金项目
国家现代农业(肉牛)产业技术体系专项经费 （肉牛）
公益性农业(肉牛)行业专项(nyhyzx07-035) （肉牛）
动物营养国家重点实验室项目. （）
