中国畜牧兽医2011，Vol.38Issue(11)：73-76,4.
转人乳铁蛋白基因奶牛多重PCR检测方法的研究
Multiplex PCR Detection of Exogenous Gene in hLF Transgenic Cow
摘要
Abstract
For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry & exit animal quarantine, detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study. The endogenous mtDNA gene specific for cow identification, exotic hLF gene,marker NPTⅡ gene were amplified according to different primers. The process of the multiplex PCR reaction was optimized. The system described herein represent simple, accurate, and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that can be reliably used for the identification of specific lines of transgenic cows.关键词
转基因奶牛/多重PCR/hLF基因Key words
transgenic cow/multiplex PCR/ hLF gene分类
生物科学引用本文复制引用
朱振营,张书霞,李风翠,林祥梅,吴绍强,仇松寅,李宁,王建武,薛振华,刘建..转人乳铁蛋白基因奶牛多重PCR检测方法的研究[J].中国畜牧兽医,2011,38(11):73-76,4.基金项目
转基因重大专项课题转基因产品检测新技术研究(2009ZX08012-001B). （2009ZX08012-001B）
