中国畜牧兽医Issue(12)：57-61,5.
氯丙嗪对大鼠离体培养卵巢颗粒细胞凋亡的影响
Effect of Chlorpromazine on Apoptosis of Rat Ovary Granulose Cells in Vitro
摘要
Abstract
The aim is to investigate the relationship between female gonad toxicity of chlorpromazine on rat and the apopto-sis of granulosa cells. Immature ovarian granulosa cell of Wistar rat was primary cultured, cells were cultured and treated with different concentration chlorpromazine(0, 0. 1, 1, 10 μmol/L) for 24 h. After exposured to chlorpromazine 24 h, cell viability was detected by MTT assay, apoptotic cells were identified -microscopically by chroma tin condensation and nuclear fragmentation with Hoechest 33258, mRNA expressions of Bax, Bcl-2 and P53 were determined by reverse transcriptase-polymerase Chain reaction (RT-PCR). Compared to control group, chlorpromazine could inhibit the proliferation of ovarian granulosa cell (P＜0. 01) in a concentration-dependent way. RT-PCR detection showed that chlorpromazine upregulated the mRNA expression of Bax, P53 and the mRNA ratio of Bax/Bcl-2. Compared with control group, the differences between relevant indicators was statistically significant(P＜0. 01) except Bax and Bax/Bcl-2 of low group. Chlorpromazine may serve as an important endocrine disrupting medicine, mediating rat granulose cell apoptosis in vitro. The effect is bidirectional and concentration dependent.关键词
氯丙嗪/卵巢颗粒细胞/细胞活力/细胞凋亡Key words
chlorpromazine/ ovary granulosa cells/cell viability/ apoptosis分类
生物科学引用本文复制引用
邬静,袁慧,文利新,彭双清..氯丙嗪对大鼠离体培养卵巢颗粒细胞凋亡的影响[J].中国畜牧兽医,2011,(12):57-61,5.基金项目
国家科技重大专项"重大新药创制"(2009ZX09501-034) （2009ZX09501-034）
