中国畜牧兽医Issue(12)：67-70,4.
猪繁殖与呼吸综合征病毒2b基因的克隆和原核表达
Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
A pair of specific primers of porcine reproductive and respiratory syndrome virus (PRRSV) 2b were designed and synthesized according to the published gene sequence of PRRSV from GenBank,and 26 gene of PRRSV was obtained by RT-PCR method. The RT-PCR product was cloned into pMD18-T vector and sequenced. After the positive gene was cloned into the prokaryotic expression vector and designated pGEX-4T-2b,the recombinant plasmid was transformed into host cell BL21 (DE3) and induced by IPTG. SDS-PAGE and Western blotting test confirmed that the expressed fusion protein is about 35 ku, which had immunogenically reactive activity.关键词
猪繁殖与呼吸综合征病毒/2b蛋白/克隆/表达Key words
porcine reproductive and respiratory syndrome virus/2b protein/clone/ expression分类
生物科学引用本文复制引用
何斌,白雪,梅林,马德慧,张社民,李勇,赵建增,高英杰,夏志平,王泽岩,刘雯,张宁,屈雪琪..猪繁殖与呼吸综合征病毒2b基因的克隆和原核表达[J].中国畜牧兽医,2011,(12):67-70,4.基金项目
运城市科技计划项目. （）
