中国畜牧兽医Issue(12)：96-100,5.
抗菌肽Shiva1a基因真核表达载体的构建及序列分析
Construction of Eukaryotic Expression Vector and Sequence Analysis of Antimicrobial Peptide Gene Shiva 1a
摘要
Abstract
To explore the effect mechanism of cecropin-class lytic peptide at early stage of infection. The mat peptide of Shiva la was amplified by overlap extension PCR and the C-terminus contained 6×His-marker. The gene sequence were recombi-nant with eukaryotic expression vector pIRES2-EGFP. After being identified by restriction enzyme digestion and sequencing, the recombinant plasmid pIRES2-EGFP-Shiva la was transfected into CHO-K1 cells by liposomes. The expression of the the recombinant plasmid pIRES2-EGFP-Shiva 1a was observed by fluorescence microscope. At the same time, the secondary structure and 3D structure were predicted by bioinformatics tools. The results lay the foundation in research of antimicrobial activities and applications of the antimicrobial peptide Shiva la in breeding for disease resistance of animals.关键词
抗菌肽/Shiva 1a/真核表达/序列分析Key words
antimicrobial peptide/ Shiva las eukaryotic expression/ sequence analysis分类
生物科学引用本文复制引用
宫晓炜,郑福英,蔺国珍,曹小安,王光华,周继章,才学鹏..抗菌肽Shiva1a基因真核表达载体的构建及序列分析[J].中国畜牧兽医,2011,(12):96-100,5.基金项目
转基因生物新品种培育科技重大专项(2009ZX08008-010B). （2009ZX08008-010B）
