中国畜牧兽医Issue(12)：122-125,4.
五华鸡J亚群白血病病毒env部分基因的和序列分析
DNA Cloning and Sequence Analysis of Partial env Gene of Avian Leukosis Virus Subgroup J in Wuhua Chicken Flocks
摘要
Abstract
The partial sequence of avian leukosis virus subgroup J (ALV-J) env gene was cloned by PCR in Anhui province, which was named as ALV-J-envl. And we compared ALV-J-envl with the partial sequence of eight ALV-J env genes in the GenBank database. The results showed that ALV-J infection in Wuhua chicken was found in Anhui province. Alignment of nine gene sequences of ALV-J env, the homology between ALV-J-envl and the other sequences ranged from 94. 2% to 96. 6%. The result revealed that the env gene from different strains had the mutation at nucleotide sequence. However, the highest homology with ALV-J-envl were HQ425636 and HM235665 gene sequences isolated from strains in China, and they gathered together in the evolutionary tree, indicating a close genetic relationship, and they evolved from common strains. The lowest homology with ALV-J-envl was the gene sequence of AY312965 isolated from Malaysia, it could be further confirmed by phylogenetic analysis.关键词
禽白血病病毒J亚群/env部分基因/序列分析Key words
avian leukosis virus subgroup J/partial env gene/sequence analysis分类
生物科学引用本文复制引用
戴银,张丹俊,胡晓苗,赵瑞宏..五华鸡J亚群白血病病毒env部分基因的和序列分析[J].中国畜牧兽医,2011,(12):122-125,4.基金项目
国家蛋鸡产业技术体系项目(CARS-41) （CARS-41）
安徽省科技攻关项目(11010302119) （11010302119）
院长青年创新基金项目(11B0418) （11B0418）
