中国畜牧兽医Issue(12)：232-235,4.
鸭疫里氏杆菌液体发酵培养工艺研究
Research in the Liquid Fermentation Technology of Riemerella anatipestifer
杨灵芝 1李本科 2马景霞 2李香云1
作者信息
- 1. 山东滨州博莱威生物技术有限公司,山东滨州 256606
- 2. 山东滨州畜牧兽医局,山东滨州 256600
- 折叠
摘要
Abstract
The cultivation of GN52 strain of Riemerella anatipestifer (RA) in improved yeast medium using liquid fermentation devices was researched. Explore Riemerella anatipestifer's growth rhythm, culture method, the optimum time for acquisition as well as the best condition for inactivation via coloration and microscopic examination, viable count, measurement of D450nm and pH value, etc. The results indicated that during the cultivation of RA the pH value stayed stable; the number of viable bacteria peaked at 320 million-350 million CFU/mL after fermenting for 20 hours at 37 ℃ ; inactivate Ag for 18 hours at 37 ℃ by 0. 3% methanal was optimums there was kind of functional relation between D450nm and the number of viable bacteria, the latter could be reckoned through measuring D450nm of RA liquid culture medium.关键词
鸭疫里氏杆菌/发酵培养Key words
Riemerella anatipestifer/ fermentation culture分类
农业科技引用本文复制引用
杨灵芝,李本科,马景霞,李香云..鸭疫里氏杆菌液体发酵培养工艺研究[J].中国畜牧兽医,2011,(12):232-235,4.
