中国畜牧杂志2011，Vol.47Issue(11)：33-37,5.
不同油脂对瘤胃原虫、细菌蛋白质及DNA影响的研究
Effects of Different Oils on the Protein and DNA of Ruminal Protozoa and Bacteria in Vitro
摘要
Abstract
The objectives of this study were to investigate the effects of different oils on the protein and DNA rf ruminal protozoa and bacteria in vitro. 3 Xuhuai White Goats with permanent cannulas were used to provide rumen liquor, in vitro culture were carried out by adding peanut oil, rapeseed oil, corn oil, and soybean oil respectively, with a set of control group (no oil). The results showed that, except for bacterial protein, no significant differences were found in the mean value of bacterial DNA, protozoal protein, protozoal DNA, microbial protein, microbial DNA, and protozoa to bacteria ratio (P>0.05), while significant differences were detected in all above indexes between sampling points according to culture time (P<0.05 or P<0.01). Additionally, the levels in turn of microbial protein and DNA were similar, there were, soybean oil > corn oil > peanut oil >control group > rapeseed oil; Whereas, protozoa to bacteria ratio of control group was the highest, followed by that of rapeseed oil, and protozoa to bacteria ratio in corn oil, or soybean oil, were relatively lower. Furthermore, correlation analysis showed that, good correlations existed between bacterial protein and DNA, also between microbial protein and DNA (2-tail<0.05).关键词
油脂/原虫/细菌/DNA/蛋白质/山羊Key words
oil/protozoa/bacteria/DNA/protein/goat分类
农业科技引用本文复制引用
拟豪杰,钱金花,李丹枫,王梦芝..不同油脂对瘤胃原虫、细菌蛋白质及DNA影响的研究[J].中国畜牧杂志,2011,47(11):33-37,5.基金项目
国家自然科学基金(37072051) （37072051）
扬州大学青年教师培育基金(2010CXJ054) （2010CXJ054）
扬州大学2010大学生科技创新基金 （）
