中国循证儿科杂志2012，Vol.7Issue(2)：136-140,5.DOI:10.3969/j.issn.1673-5501.2012.02.012
儿童EB病毒相关噬血细胞淋巴组织细胞增生症NK细胞表面受体和CD107a表达
Expression of NK cell surface receptors and CD107a in Epstein-Barr virus associated hemophagocytic lymphohistiocytosis children
摘要
Abstract
Objectives The role of NK cell function as an etiological or prognostic factor in EBV-HLH remains unclear. The present study tried to investigate the expression of NK cell surface activating receptors and CD107a in EBV-HLH patients. Methods EBV-HLH patients and age- and sex-matched normal controls were selected from August 2009 to May 2011 in Beijing Children's Hospital. Multicolored immunofluorescence flowcytometric analysis was performed to detect NK cellsurface activating receptors, perform and IL-2 stimulated NK cells in peripheral blood from EBV-HLH patients and controls. Results Eight cases were enrolled in EBV-HLH group and 8 normal children were enrolled in control group. ① There was no statistical difference between EBV-HLH patients and normal controls in NK cell surface activating receptors ( NKP30, NKP46, NKG2D, DNAM-1 and 2B4 ), perform or basic CD107a expression ( P>0. 05 ). ②There was no statistical difference in basic CD107a, CD107a/K562, CD107a/2B4/P815, CD107a/NKG2D/P815 or CD107a/2B4/NKG2D/P815 expression between EBV-HLH group and control group. ③Basic CD107a expression, CD107a/K562 and CD107a/NKG2D/P815 expression in normal controls significantly changed48 hours after IL-2 stimulation, while such difference was not seen in EBV-HLH patients ( P >0.05 ). Conclusions The present results indicated that there may be some defects in responding to IL-2 in EBV-HLH patients.关键词
NK细胞/EB病毒/噬血细胞淋巴组织细胞增生症/儿童/病例对照研究Key words
NK cell/Epstein-Barr virus/Hemophagocytic lymphohistiocytosis/Child/Case-control study引用本文复制引用
秦强,谢正德,刘春艳,黄志卓,王亚丽,张寒,赵晓曦,申昆玲..儿童EB病毒相关噬血细胞淋巴组织细胞增生症NK细胞表面受体和CD107a表达[J].中国循证儿科杂志,2012,7(2):136-140,5.基金项目
国家自然科学基金:81171568 （）
北京市自然科学基金:7113152 （）
中国博士后科学基金:20090460396 （）
北京市卫生系统高层次卫生技术人才专项经费(学科骨干培养计划):2009-3-42 （学科骨干培养计划）
