中国药房2011，Vol.22Issue(47)：4489-4490,2.
HPLC法测定江西不同产地蔓荆子中蔓荆子黄素的含量
Content Determination of Vitexicarpin in Vitex trifolia from Different Producing Areas of Jiangxi Province by HPLC
杨人泽 1钟美兴 1钟星明1
作者信息
- 1. 赣南医学院第一附属医院,赣州市341000
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of vitexicarpin in Vitex trifolia from different producing areas of Jiangxi province. METHODS: HPLC method was adopted. The determination was performed on Alltima HP C18 (250 mm ×4.6 mm,5 μm) column with the mobile phase consisted of acetonitrile-50 mmol·L-1 potassium dihydrogen phosphate solution (pH value adjusted to 3.0 with phosphoric acid, 50:50), at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 254 nm, and the column temperature was room temperature. The injection volume was 10 μL. RESULTS: The linear range was 0.2~1.2 μg(r=0.999 4) with an average recovery of 99.96% (RSD= 1.42%, n=9).Vitexicarpin contents of V. trifolia from different areas differed greatly. CONCLUSION: The method is simplp and rapid with good linear relationsgip, which can be used for the content determination of vitexicarpin in V. trifolia.关键词
蔓荆子/蔓荆子黄素/高效液相色谱法/含量测定Key words
Vitex trifolia/ Vitexicarpin/ HPLC/ Content determination分类
医药卫生引用本文复制引用
杨人泽,钟美兴,钟星明..HPLC法测定江西不同产地蔓荆子中蔓荆子黄素的含量[J].中国药房,2011,22(47):4489-4490,2.
