中国药房2011，Vol.22Issue(48)：4594-4596,3.
HPLC法同时测定双黄连片中黄芩苷、绿原酸、连翘苷、连翘酯苷的含量
Content Determination of Baicalin, Chiorogenic Acid, Forsythin and Forsythoside A in Shuanghuanglian Tablets by HPLC
陈国宝 1宋桂萍 1杨弃尘1
作者信息
- 1. 南京中医药大学江阴附属医院,江阴市214400
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for simultaneously determination of baicalin, chlorogenic acid, forsythin and forsythoside A in Shuanghuanglian tablets. METHODS: HPLC method was carried out on a Hedera ODS 2 column with mobile phase consisted of methanol-0.2% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was 324 tun and 280 nm. Injection volume was 10 μL and column temperature was 30 t .RESULTS: The linear ranges of baicalin, chlorogenic acid, forsythin, Forsythosid A were in the range of 28.525～342.3 ng·mL-1 (r=0.999 9), 4.56～54.72 ug·mL-1(r=0.999 5), 1.55～18.6 ug·mL-1(r=0.999 4), 1.81～21.72 ug·mL-1(r= 0.999 6), respectively. The average recoveries of four active ingredients were 98.12%, 96.99%, 103.16% , 100.14% , respectively (n=6). CONCLUSION: The method is simple, feasible and very suitable for the quality control of Shuanghuanglian tablets.关键词
双黄连片/双波长/高效液相色谱法/含量测定Key words
Shuanghuanglian tablets/Double wavelength/HPLC/Content determination分类
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陈国宝,宋桂萍,杨弃尘..HPLC法同时测定双黄连片中黄芩苷、绿原酸、连翘苷、连翘酯苷的含量[J].中国药房,2011,22(48):4594-4596,3.
