中国药房2012，Vol.23Issue(1)：34-36,3.DOI:10.6039/j.issn.1001-0408.2012.01.12
丙二酸次氮酰胺对刀豆蛋白刺激的胶原性关节炎模型大鼠CD4+CD25+调节性T淋巴细胞体外分化的影响
Effect of Malonic Acid Amide Nitrogen on the Differentiation of CD4+CD25+ Tregs of ConA-induced CIA Rats in Vitro
王婷玉 1李俊 2金芝贵 1吴飞华 1王小华 2周倩2
作者信息
- 1. 上海交通大学医学院附属第九人民医院药剂科,上海200011
- 2. 安徽医科大学药学院,合肥230032
- 折叠
摘要
Abstract
OBJECTIVE: To study the effect and mechanism of active metabolite malonic acid amide nitrogen (A771726) on the differentiation of CD4+CD25+ Tregs from collagen-induced arthritis (CIA) rats in vitro. METHODS: Rats were divided into normal control group and CIA model group. The splenocyte suspension was prepared after modeling then divided into normal group, model group and A771726 low-dose, medium-dose and high-dose groups (0.1,1,10 μmol·L-1). Those groups were cultured in culture solution of ConA and relevant medicine for 48 h. The proliferation of spleen lymphocytes from CIA rats was checked with MTT assay; immunofluorescence flow cytometry was used to detect the proportion of CD4+CD25+ Tregs in CD4+T; RT-PCR was used to detect the expression of Foxp3 mRNA and TGF-β mRNA; ELISA was used to detect the content of TGF-p. RESULTS: Compared with normal group, the proliferation of ConA-induced splenocytes of CIA rats in model group increased significantly (P<0.01), while the proportion of CD4+CD25+ Tregs, the expression of Foxp3 and TGF-P mRNA and the content of TGF-P decreased significantly (P<0.05). Compared with model group, the proliferation of ConA-induced splenocytes in A771726 groups decreased significantly (P<0.05 or P<0.01), while the proportion of CD4+CD25+ Tregs in CD4+T of A771726 high-dose group increased significantly, and the expression of Foxp3 and TGF-P mRNA and the content of TGF-P in A771726 medium-dose and high-dose groups increased significantly (P<0.05 or P<0.01). CONCLUSION: A771726 could inhibit the proliferation of splenocytes of CIA rats, and facilitate the differentiation of CD4+CD25+Tregs, which may be related to the up-regulation of the expression and secretion of TGF-β.关键词
CD4+CD25+调节性T淋巴细胞/来氟米特/活性代谢物/丙二酸次氮酰胺/胶原性关节炎/大鼠/转化生长因子βKey words
CD4+CD25+ Tregs/ Leflunomide/ Active metabolite/ Malonic acid amide nitrogen/ Collagen-induced arthritis/ Rats/ TGF-β分类
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王婷玉,李俊,金芝贵,吴飞华,王小华,周倩..丙二酸次氮酰胺对刀豆蛋白刺激的胶原性关节炎模型大鼠CD4+CD25+调节性T淋巴细胞体外分化的影响[J].中国药房,2012,23(1):34-36,3.
