中国药房2012，Vol.23Issue(3)：215-217,3.DOI:10.6039/j.issn.1001-0408.2012.03.08
关苍术超临界提取物对乙醇致大鼠胃黏膜损伤保护作用的研究
Protective Effects of Supercritical CO2 Extract from Atractylodes japonica on Ethanol-induced Gastric Mucosal Injury in Rats
刘男 1廉丽花 1张颖丽 1张善玉1
作者信息
- 1. 长白山生物资源与功能分子教育部重点实验室,吉林延吉133002
- 折叠
摘要
Abstract
OBJECTIVE: To study the protective effects of supercritical CO2 extract from Atractylodes japonica (GCT)against ethanol-induced gastric mucosal injury in rats. METHODS: 40 Wistar rats were divided into 5 groups, I.e. Normal control group (constant volume distilled water) , model group (constant volume distilled water) , hydrotalcite group (0.37 g ? Kg"1) , GCT high-dose and low-dose groups (0.70,0.35 g-kg"1).The rat model of acute gastric damage was induced by absolute ethanol (0.5 mL? 100 g"1) intragastrically. Those groups were given relevant medicine intragastrically once a day for 5 days. Model was induced 30 min after the last medication. The gastric mucosal injury area was observed. The contents of MDA and NO, the activity of SOD, MPO and NOS, and the expression of COX-2 in gastric mucous were determined. RESULTS: High-dose and low-dose of GCT suppressed the elevation of MDA and MPO, and enhanced the SOD activity, the decrease of NO content and NOS activity. Moreover, GCT significantly reduced COX-2 expression. CONCLUSION: GCT can prevent ethanol-induced gastric mucosal injury, partly through scavenging free radical, maintaining NO level and down-regulating COX-2.关键词
关苍术/乙醇/胃黏膜损伤/环氧化物酶2Key words
Atractylodes japonica/ Ethanol/ Gastric mucosal injury/ COX-2分类
医药卫生引用本文复制引用
刘男,廉丽花,张颖丽,张善玉..关苍术超临界提取物对乙醇致大鼠胃黏膜损伤保护作用的研究[J].中国药房,2012,23(3):215-217,3.
