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超高效液相色谱法测定人参中人参皂苷Re的含量

孙全乐 蔡广知 贡济宇

中国药房2012,Vol.23Issue(3):258-260,3.
中国药房2012,Vol.23Issue(3):258-260,3.DOI:10.6039/j.issn.1001-0408.2012.03.25

超高效液相色谱法测定人参中人参皂苷Re的含量

Content Determination of Ginsenoside Re in Panax ginseng by UPLC

孙全乐 1蔡广知 2贡济宇2

作者信息

  • 1. 长春医学高等专科学校,长春130031
  • 2. 长春中医药大学,长春130117
  • 折叠

摘要

Abstract

OBJECTIVE: To develop the method for the content determination of ginsenoside Re in Panax ginseng. METHODS: UPLC method was adopted. The determination was performed on Waters patened hybrid particle column (50 mmx2.1 mm, 1.7 urn) with mobile phase consisted of acetonitreile-water (19:81) with detection wavelength at 203 nm. RESULTS: The linear range of ginsenoside Re was 0.531-1.239 (ig-mL"1(r=0.999 8) with an average recovery of 98.70%(RSD=1.23%,n=6). CONCLUSION: The method is simple, convenient and accurate with high efficacy, and it can be used for the content determination and quality control of P. Ginseng.

关键词

人参/人参皂苷Re/超高效液相色谱法/含量测定

Key words

Panax ginseng/ Ginsenoside Re/ UPLC/ Content determination

分类

医药卫生

引用本文复制引用

孙全乐,蔡广知,贡济宇..超高效液相色谱法测定人参中人参皂苷Re的含量[J].中国药房,2012,23(3):258-260,3.

中国药房

OACSCDCSTPCD

1001-0408

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