中国药房2012，Vol.23Issue(5)：437-439,3.DOI:10.6039/j.issn.1001-0408.2012.05.19
微生物浊度法与管碟法测定乙酰麦迪霉素制剂的效价
Comparison of Antimicrobial Activity of Acetyl Midecamycin Preparations by Turbidimetric Method and Cylinder-plate Method
摘要
Abstract
OBJECTIVE: To establish turbidimetric method for the antimicrobial activity of Acetyl midecamycin preparations (tablet and suspension) , and to compare turbidimetric method with cylinder-plate method. METHODS: The test organism was Staphylococcus aureus with adding amount of 1.2% and cultured at (37 ± 0.5) °C for about 3~4 h. The detection wavelength was set at 530 nm. The antimicrobial activity of tablets and suspension detected by turbidimetric method were compared with those by cylinder-plate method. RESULTS: The liner range of acetyl midecamycin was 0.3~3.0 u·mL-1 (r=0.998 3). Average recoveries of tablets and suspension were 101.99% (RSD=1.94%, w=9) and 101.68% (RSD=1.68%, n=9). There was no significant difference between 2 kinds of methods (?=1.473<4.303). CONCLUSION: Compared with cylinder-plate method, turbidimetric method is accurate, reliable, rapid with lower detection limit and less influencing factors. Both methods can be used for detecting acetyl midecamycin preparations.关键词
乙酰麦迪霉素/片剂/千混悬剂/效价/浊度法/管碟法Key words
Acetyl midecamycin/ Tablet/ Suspension/ Antimicrobial activity/ Turbidimetric method/ Cylinder-plate method分类
医药卫生引用本文复制引用
王淑娟,高燕霞,程桂茹..微生物浊度法与管碟法测定乙酰麦迪霉素制剂的效价[J].中国药房,2012,23(5):437-439,3.基金项目
河北省2006年科学技术研究与发展指导计划(06276445) （06276445）
