中国药房2012，Vol.23Issue(5)：440-442,3.DOI:10.6039/j.issn.1001-0408.2012.05.20
辐照对呋塞米注射液含量的影响考察
Effects of Irradiance on the Content Changes of Furosemide Injection
徐靖 1卢华 2管海燕 1向志红 1卢来春1
作者信息
- 1. 第三军医大学大坪医院野战外科研究所药剂科,重庆400042
- 2. 山东即墨市中医院妇产科,山东即墨266000
- 折叠
摘要
Abstract
BJECTIVE: To develop a method for the content determination of furosemide in Furosemide injection, and to study the content changes of Furosemide injection after absorbing y-ray irradiance in different intensity levels. METHODS: HPLC method was adopted. The determination was performed on Diamonsil Technology C18 column with mobile phase consisted of metha-nol-0.1% phosphoric acid (60:40) at the flow rate of 1 mL·mu-1. The detection wavelength was set at 286 nm. Same batch of Furosemide injection was prepared into 20 μg·mL-1 solution and then put in the 60Co-y ray. The content of furosemide was determined after absorbing the radiation dose of 1, 2, 4, 8, 16, 25 kGy for one time. RESULTS: The linear range of furosemide was 6~24 μg·mL-1 (r=0.999 7) with low, medium and high concentrations average recoveries of 100.15% , 101.52% and 101.35% ; and inter-day and intra-day RSD were less than 2% ; the content changes of injection had more difference at irradiance dose of above 2 kGy. The contents of furosemide were 18 μg·mL-1, 17 μg·mL-1, 14 μg·mL-1, 11 μg·mL-1 and 7 μg·mL-1 at irradiance doses of 2, 4, 8, 16, 25 kGy, respectively. The increase of irradiance density showed a linear relationship with the decrease of content (r= 0.986 2). CONCLUSION: The developed HPLC can be used for the content determination of Furosemide injection; and the irradiance has a significant impact on the stability of Furosemide injection.关键词
呋塞米注射液/辐照/含量测定/高效液相色谱法Key words
Furosemide injection/ Irradiance/ Content determination/ HPLC分类
医药卫生引用本文复制引用
徐靖,卢华,管海燕,向志红,卢来春..辐照对呋塞米注射液含量的影响考察[J].中国药房,2012,23(5):440-442,3.
