中国药房2012，Vol.23Issue(6)：528-529,2.DOI:10.6039/j.issn.1001-0408.2012.06.17
RP-HPLC法测定人血清中舒芬太尼的浓度
Determination of Sufentanil Concentration in Human Serum by RP-HPLC
谢瑞祥 1范芳 1张莲莲 1杨明华2
作者信息
- 1. 福建省肿瘤医院药理研究室,福州350014
- 2. 福建省肿瘤医院麻醉科,福州 350014
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the determination of sufentanil concentration in human serum. METHODS: Sufentanil was extracted from serum by ethyl ethanoate-petroleum ether(4:1) and determined by RP-HPLC. The determination was performed on Symmetry Cu column with mobile phase consisted of acetonitrile-0.02 mol-L"1 NH4Ac-HCL(pH4.3) (45: 55). The detection wavelength was set at 230 run and the column temperature was room temperature. Estazolam was used as internal standard. RESULTS: The linear range of sufentanil was 0.025-4.000 ug-mL"'(r=0.999 9) with intra-day and inter-day RSDs of 3.49%-4.51% and 4.67%-5.99%. Average recoveries were 101.92% -107.04%. CONCLUSION: The method is simple, rapid, accurate and specific with few steps. It is suitable for drug concentration monitoring of sufentanil in human serum.关键词
反相高效液相色谱法/舒芬太尼/血药浓度/药动学Key words
RP-HPLC/ Sufentanil/ Serum concentration/ Pharmacokinetics分类
医药卫生引用本文复制引用
谢瑞祥,范芳,张莲莲,杨明华..RP-HPLC法测定人血清中舒芬太尼的浓度[J].中国药房,2012,23(6):528-529,2.
