中国药房2012，Vol.23Issue(7)：629-632,4.DOI:10.6039/j.issn.1001-0408.2012.07.19
不同产地甘木通药材多谱图鉴定及质量控制
Multiple Chromatograms Identification and Quality Control of Clematis filamentosa from Different Producing Areas
陈道阳 1孙平川 2黄健玲 3邓亚利3
作者信息
- 1. 广东省人民政府机关门诊部,广州 510030
- 2. 陕西省中医药研究院,西安 710003
- 3. 华南农业大学制药工程系,广州 510642
- 折叠
摘要
Abstract
OBJECTIVE: To establish the TLC, HPLC, FTIR and GC-MS fingerprints of Clematis filamentosa for quality control of it. METHODS: HPLC method was used to determine the content of kaempferide. The determination was performed on Waters Xbridge C18(250 mm×4.6 mm, 5 urn) column with mobile phase consisted of acetonitrile-0.4% acetic acid (gradient elution). The detection wavelength was set at 326 nm; Developer of ligroin-methanol (9:1) and chloroform-methanol(9: l)were used in lig-roin extract and acetic ether extract respectively in TLC. Absorption peaks were labeled in petroleum ether and ethyl acetate by FTIR. The volatile oil components were analyzed by GC-MS. RESULTS: The common characteristic peaks in TLC,HPLC and IR fingerprints of C. filamentosa were used for qualitative analysis; the content of kaempferide was measured by HPLC; 37 components were identified by GC-MS. CONCLUSION: Multiple chromatograms can be used for comprehensive identification and quality control of C. filamentosa.关键词
甘木通/高效液相色谱法/山柰素/薄层色谱法/傅里叶红外光谱法/气相色谱-质谱联用法/多谱图体系Key words
Clematis filamentosa/ HPLC/ Kaempferide/ TLC/ FTIR/ GC-MS/ Multiple chromatograms system分类
医药卫生引用本文复制引用
陈道阳,孙平川,黄健玲,邓亚利..不同产地甘木通药材多谱图鉴定及质量控制[J].中国药房,2012,23(7):629-632,4.
