中国药房2012，Vol.23Issue(7)：642-643,2.DOI:10.6039/j.issn.1001-0408.2012.07.24
紫外分光光度法测定甘草中总黄酮的含量
Content Determination of Total Flavonoids from Radix Et Rhizoma Glycyrrhizae by UV Method
高宏 1贠亚波 1兀江波 1曹峡萍1
作者信息
- 1. 三门峡市中医院药剂科,河南 三门峡 472000
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae. METHODS: Liquiritin and 10% KOH solution were used as substance control and color reagent to optimize color developing time and amount of color reagent. At last, UV spectrophotometry was established for the content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae. RESULTS: Optimal amount of color reagent was 1.5 mL and color developing time was 15 min; the linear range of liquiritin was 0.012-0.030 mg-mL"1(r=0.999 8) with an average recovery of 99.0% (RSD= 2.60, n=9). CONCLUSION: The method is rapid, simple and effective, and it is suitable for the content determination of content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae.关键词
甘草/总黄酮/含量测定/紫外分光光度法/甘草苷Key words
Radix Et Rhizoma Glycyrrhizae/ Total flavonoids/ Content determination/ UV spectrophotometry/ Liquiritin分类
医药卫生引用本文复制引用
高宏,贠亚波,兀江波,曹峡萍..紫外分光光度法测定甘草中总黄酮的含量[J].中国药房,2012,23(7):642-643,2.
