中国药房Issue(8)：737-739,3.DOI:10.6039/j.issn.1001-0408.2012.08.27
HPLC双波长法同时测定复方维A酸软膏中2主药的含量
Simultaneous Content Determination of 2 Components in Compound Tretinoin Ointment by Dual Wavelength HPLC
魏立明 1宋霞 1宋三孔 1杨效宇 1焦海胜2
作者信息
- 1. 兰州大学药学院,兰州730000
- 2. 兰州大学第二医院药剂科,兰州730030
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the simultaneous determination of tretinoin and chlorpheniramine maleate in compound tretinoin ointment. METHODS: HPLC method was adopted. Luna da column was used with mobile phase consisted of methanol-phosphate buffer (0.02 mol-L-1 potassium dihydrogen phosphate, pH adjusted to 7.0 using 1 mol·L-1 sodium hydroxide, F/K=80:20) at a flow rate of 1.0 mL·min-1. The column temperature was 30 °C and injection volume was 20 μL. The detection wavelength was set at 350 nm and 262 nm for tretinoin and chlorpheniramine maleate, respectively. The content of tretinoin and chlorpheniramine maleate was determined by external standard method. RESULTS: The liner range of tretinoin was 20~100 μg·mL-1 (t-=0.999 9) with an average recovery rates of it at low, medium and high concentrations were 98.48% , 97.15%, 98.88% (RSD=0.92%). The liner range of chlorpheniramine maleate was 30-150 μg·mL-1 (r=0.999 9) with an average recovery rates of it at low, medium and high concentrations were 100.47% , 99.01% , 99.75% (RSD=0.83% ). CONCLUSION: The established method is simple, accurate and reproducible. It can be used for the quality control of compound tretinoin ointment.关键词
高效液相色谱法/双波长/复方维A酸软膏/维A酸/马来酸氯苯那敏/含量测定Key words
HPLC/ Dual wavelength/ Compound tretinoin ointment/ Tretinoin/ Chlorpheniramine maleate/ Content determination分类
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魏立明,宋霞,宋三孔,杨效宇,焦海胜..HPLC双波长法同时测定复方维A酸软膏中2主药的含量[J].中国药房,2012,(8):737-739,3.
