中国医科大学学报2011，Vol.40Issue(11)：983-985,1000,4.DOI:21-1227/R.20111128.1738.025
PTSD样大鼠中缝背核神经元细胞凋亡及其TMP活性表达变化的研究
Expression of TMP Activity and Apoptosis in Dorsal Raphe Nucleus of PTSD Rats
摘要
Abstract
Objective To study expression of TMP activity and apoptosis in the dorsal raphe nucleus of PTSD (posttraumatic stress disorder) rats. Methods The SPS-method was used to set up the rat PTSD models. Rats were randomly divided into ld,7d and 14d groups of SPS and normal group. Apoptosis in the dorsal raphe nucleus were detected by using annexin V-FTTC/PI double-labled flow cytometry. The changes of TMP activity in the dorsal raphe were detected by light microscope and transmission electron microscopy (TEM). Results SPS exposure resulted in a significant change of characteristic morphologic changes of apoptosis in the dorsal raphe nucleus. Apoptosis rate in dorsal raphe nucleus was significantly increased and TMP activity was significantly enhanced compared with that of normal control group ( P < 0.05). Both markers reached the peak at 7 d after SPS exposure. Conclusion Dorsal raphe neurons of PTSD rats have had appotosis. The changes of apoptosis rate and TMP activity enhanced enhancing may might indicate that the TMP is was involved in degradation and processing of the apoptotic product in dorsal raphe nucleus, and smaller volume of pons may might be related to appotosis of dorsal raphe nucleus.关键词
PTSD/中缝背核/神经元/细胞凋亡/TMPKey words
posttraumatic stress disorder/ dorsal raphe nucleus/ neuron/ apoptosis/ thinamine monophosphatase分类
医药卫生引用本文复制引用
刘冬娟,肖冰,王恩华,韩芳,石玉秀..PTSD样大鼠中缝背核神经元细胞凋亡及其TMP活性表达变化的研究[J].中国医科大学学报,2011,40(11):983-985,1000,4.基金项目
国家自然科学基金资助项目(81171282,31140060) （81171282,31140060）
国家教育部博士点基金资助项目(20092104110016) （20092104110016）
