中国医科大学学报2011，Vol.40Issue(11)：997-1000,4.DOI:21-1227/R.20111128.1735.003
菩人丹超微粉对糖尿病大鼠视网膜TNF-α表达的影响
Effects of Purendan Superfine Powder on Expression of Retinal Tumor Necrosis Factor-α in Diabetic Rat
董志军 1张铁民 1付笑笑 1张垒 1石晶 1郭淑玲1
作者信息
- 1. 承德医学院附属医院眼科,河北承德067000
- 折叠
摘要
Abstract
Objective To investigate the effects of Purendan superfine powder(PRD) on retinal tumor necrosis factor-a (TNF-α) expression in diabetic rat. Methods 36 male Wistar rats were randomly divided into 3 groups (n =12): normal control group, diabetes model group,PRD treatment group. The diabetes model rats were made by continuous intraperitoneal injection of Streptozotocin (STZ). After the diabetes animal model was successfully established, the rats in PRD treatment group were lavaged with PRD( 1.8 g·kg-1·d-1) for 3 months. The morphological changes of retina were observed by HE staining. Western blot were used to assess the expression of TNF-a protein in retina, reverse transcription-polvmerase chain reaction (RT-PCR) was employed to examine the expression of TNF-α mRNA in retina. Results In diabetes model rats,the retina showed obvious pathological changes,the expression of TNF-a protein and mRNA in retina were obviously higher than normal control rats (P < 0.01). Compared with diabetes model rats,the pathological changes of retina were alleviated significantly, the expression of TNF-α protein and mRNA in retina of rats in PRD treatment group decreased significantly (P < 0.01). Conclusion PRD can reduce vascular leakage and inhibit pathologic neovascularization by down-regulating TNF-α expression in retina,thus has protective effects on retinal microangiopathy during diabetes.关键词
菩人丹超微粉/糖尿病视网膜病变/肿瘤坏死因子α/大鼠Key words
Purendan superfine powder/ diabetic retinopathy/ tumor necrosis factor-a/ rat分类
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董志军,张铁民,付笑笑,张垒,石晶,郭淑玲..菩人丹超微粉对糖尿病大鼠视网膜TNF-α表达的影响[J].中国医科大学学报,2011,40(11):997-1000,4.
