中国医科大学学报2011，Vol.40Issue(12)：1092-1095,4.DOI:21-1227/R.20111226.1611.003
重组血管内皮生长因子165对肝硬化大鼠肝功能的影响
Effect of Recombinant Vascular Endothelial Growth Factor 165 on the Liver Function in Cirrhotic Rats
于良立 1赵金满 1蒋伟伟1
作者信息
- 1. 中国医科大学附属第一医院消化内科,沈阳110001
- 折叠
摘要
Abstract
Objective To investigate the effect of portal vein infusion of recombinant vascular endothelial growth factor 165 on liver function in cirrhotic rats. Methods Fifty male SD rats, weighting 180-220 g.were randomly divided into normal group (n = 10) and model group (n = 40) . The model group was used to induce cirrhosis using the thioacetamide approach. After 10 weeks, 25 cirrhotic rats were randomly divided into experimental group (n = 15) and model control group (n = 10). The experimental group was intubated for implantation of an Alzet osmotic pump, which was used to infuse recombinant VEGF165 via the portal vein for 2 weeks. The normal group and model control group underwent sham operation. All rats were killed after 2 weeks, and the pathological changes in liver tissue were observed by HE staining . Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB) and total bilirubin (TBIL) content were measured by automatic biochemical analyzer. Results Degeneration and necrosis of liver cells, diffused proliferation of fibrous connective tissue and the formation of pseudo lobules appeared in the model control group. In the experimental group, degeneration and necrosis of liver cells were milder and the rate of liver fibrosis was improved significantly compared to the model control group (P < 0.01). Compared to the normal group, levels of ALT, AST, TBIL in the serum of cirrhosis control group were significantly increased, while ALB decreased; the difference was statistically significant (P < 0.01). Levels of ALT, AST, TBIL in the serum of cirrhosis experimental group were significantly decreased, while ALB increased;the difference was statistically significant ( 115.71±12.63 U/L vs 192.36±21.84 U/L;196.26± 18.45 U/L vs 295.11 ±31.78 U/L;6.32±1.32 μmol/L vs 10.69±2.47 μmol/L;14.57±1.92g/L vs 9.90±1.27 g/L;P < 0.01). Conclusion Portal vein infusion of VEGF165 can improve the liver function in rats with cirrhosis.关键词
肝硬化/血管内皮生长因子/肝功能Key words
liver cirrhosis/ vascular endothelial growth factor/ liver function分类
医药卫生引用本文复制引用
于良立,赵金满,蒋伟伟..重组血管内皮生长因子165对肝硬化大鼠肝功能的影响[J].中国医科大学学报,2011,40(12):1092-1095,4.
