中国医科大学学报2011，Vol.40Issue(12)：1096-1098,3.DOI:21-1227/R.20111226.1612.004
MK-801及CNQX对尾末端截断后小鼠血清褪黑素含量变化的影响
Effect of MK-801 or CNQX on the Change of Serum Melatonin Contents of Mice after Amputating Extremity of the Tail
吴敏范 1代秋竹 1郭丽 2陈魁敏 3商丽宏 3杨宇1
作者信息
- 1. 沈阳医学院生理学教研室,沈阳110034
- 2. 沈阳医学院科学实验中心,沈阳110034
- 3. 沈阳医学院机能实验中心,沈阳110034
- 折叠
摘要
Abstract
Objective To study the effect of glutamic acid receptor antagonist, MK-801 or CNQX on the change of serum melatonin contents of mice after amputating extremity of the tail, which could provide new scientific experimental data for further investigating plastic changes in central nervous system after amputation. Methods The distal 2.5 cm length of the mice tail was removed as an experiment model of amputation. The blood of the mice at 2 h after the amputation was obtained by cutting the mice heads. FLISA method was used to measure the serum melatonin contents of the mice. MK-801 or CNQX was injected via the caudalis vein. Results Compared with serum melatonin contents of the mice of control groups, serum melatonin content of the mice at 2 h after the amputation was significantly decreased (P<0.05).The intravenous injection of MK-801 or CNQX antagonized the decrease in the mice's serum melatonin contents at 2 h after the amputation. Conclusion The results indicate that serum melatonin content of the mice at 2 h after amputating extremity of the mice tail is significantly decreased. The NMDA receptor and AMPA/Kainate receptor are involved in the processes of decrease in serum melatonin contents induced by amputating extremity of the mice tail.关键词
幻肢痛/N-甲基-D-天冬氨酸受体/α-氨基3-羟基5-甲基4-异恶唑丙酸/海人藻氨酸受体/褪黑素/小鼠Key words
phantom limb pain/ NMDA receptor/ AMPA/Kainate receptor/ melatonin/ mouse分类
医药卫生引用本文复制引用
吴敏范,代秋竹,郭丽,陈魁敏,商丽宏,杨宇..MK-801及CNQX对尾末端截断后小鼠血清褪黑素含量变化的影响[J].中国医科大学学报,2011,40(12):1096-1098,3.
