中国医科大学学报2011，Vol.40Issue(12)：1099-1102,4.DOI:21-1227/R.20111226.1612.005
HPC2突变体真核表达载体的构建
Constructionof Mutated HPC2 on Eukaryotic Expression Vector
何菲 1刘鲜艳 1吴炜 1姜勇 2赵明1
作者信息
- 1. 南方医科大学病理生理教研室,广东省休克微循环重点实验室,广州510515
- 2. 南方医科大学病理生理教研室,广东省功能蛋白质组学重点实验室,广州510515
- 折叠
摘要
Abstract
Objective To construct mutated HPC2 eukaryotic expression vector and to express it in U2OS cells. Methods By means of Invitrogen Gateway system, wild type HPC2 cDNA was obtained from U2OS cells mRJMA by RT-PCR, and constructed into pcDNA 3.2/V5 vector. The expression of HPC2 was confirmed by western blot. Mutated HPC2 were made according to phosphorylau'on site prediction software analysis (NetPhosKl .0) and constructed with a mutation kit (TaKaRa MutanBEST). Results Double restriction enzyme digestion and DNA sequencing testified the correct sequence and direction of mutated HPC2/V5 eukaryotic expression vector. Conclusion The successfully constructed mutated HPC2/V5 eukaryotic expression vector could provide a beneficial tool for the research of HPC2/V5.关键词
HPC2/基因表达/突变/U2OS细胞Key words
HPC2/ mutation construction/ U2OS分类
生物科学引用本文复制引用
何菲,刘鲜艳,吴炜,姜勇,赵明..HPC2突变体真核表达载体的构建[J].中国医科大学学报,2011,40(12):1099-1102,4.
